tailieunhanh - Probitoic properties of lactobacillus strains from fermented food and infant feaces

The results for cell surface hydrophobicity and susceptibility against antibiotics also showed that L. fermentum JMC 7776 and L. plantarum P-8 had higher cell surface hydrophobicity than the rests. All Lactobacillus tested were resistant to vancomycin and susceptible to streptomycin. The results obtained in this investigation will be used to select potentially probiotic strains for in vivo study. | Journal of Science and Technology 54 (5) (2016) 632-642 DOI: PROBITOIC PROPERTIES OF LACTOBACILLUS STRAINS FROM FERMENTED FOOD AND INFANT FEACES Le Nguyen Thi My1, Huong Nguyen Thuy2 1 2 Department of Fishery, HCMC University of Food Industry, 140 Le Trong Tan Street, Tay Thanh Ward, Tan Phu District, Ho Chi Minh City, Vietnam Department of Chemical Engineering, HCMC University of Technology, 268 Ly Thuong Kiet, Ward 14, District 10, Ho Chi Minh City, Vietnam * Email: mylethang81@ Received: 22 December 2015; Accepted for publication: 2 April 2016 ABSTRACT Lactobacillus strains are a major part of the probiotics, microflora of the intestine and of fermented foods. The aim of this study was to evaluate the potential probiotics of six Lactobacillus strains (L. fermentum 39-183; L. plantarum P-8; L. casei ATCC 334; L. rhamnosus ATCC 8530, L. brevis KB 290 and L. fermentum JMC 7776). Probiotic properties such as acid tolerance, bile resistance, bacteriocin-like activity, cell surface hydrophobicity and antibiotic resistance were assessed. In vitro results obtained showed that all Lactobacillus strains tested were able to meet the basic requirements for probiotic functions as they demonstrated probiotic characteristics such as tolerance to pH and 2% bile salt. All Lactobacillus strains inhibited the growth of E. coli, Staphylococcus aureus and Salmonella Typhi. Among strains tested, L. plantarum P-8 showing inhibitory is very promising with inhibition zone ranging between to mm. The results for cell surface hydrophobicity and susceptibility against antibiotics also showed that L. fermentum JMC 7776 and L. plantarum P-8 had higher cell surface hydrophobicity than the rests. All Lactobacillus tested were resistant to vancomycin and susceptible to streptomycin. The results obtained in this investigation will be used to select potentially probiotic strains for in vivo .