tailieunhanh - Optimization of enzymatic hydrolysis conditions for increasing the efficiency of dry matter extracted from Limonia acidissima pulp by combined cellulase- pectinase enzymes using response surface methodology
At the conditions, the efficiency of the prediction model reached % and it had no significant difference compared with experimental value ( %). That increased % compared with the efficiency from the non-enzymatic extraction. Besides, the recovery efficiency of carbohydrate reached %. Further, the content of extracted phenolic, carotenoids and DPPH and ABTS radical scavenging activity highly increased, which reached mg GAE, mg, and mg trolox equivalent antioxidant capacity (TEAC) from 100 g L. acidissima pulp, respectively. | Journal of Science and Technology 55 (1) (2017) 15-28 DOI: OPTIMIZATION OF ENZYMATIC HYDROLYSIS CONDITIONS FOR INCREASING THE EFFICIENCY OF DRY MATTER EXTRACTED FROM Limonia acidissima PULP BY COMBINED CELLULASE -PECTINASE ENZYMES USING RESPONSE SURFACE METHODOLOGY Pham Bao Nguyen1, *, Dong Thi Anh Dao2 1 Postharvest Technology center, Tra Vinh University, 126 Nguyen Thien Thanh street, Ward 5, Tra Vinh city, Vietnam 2 Dept. Food Technology, Vietnam Nat. Uni. HCM University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, HCMC, Vietnam * Email: pbnguyen@ Received: 27 November 2015; Accepted for publication: 1 December 2016 ABSTRACT The Limonia acidissima (L. acidissima) fruits are rich in nutrient values and bioactive compounds. The hydrolysis of L. acidissima pulp was researched by combined cellulase and pectinase enzymes to increase the yield of dry matter and bioactive compounds. In this study, the hydrolysis conditions by using the combined enzymes for increasing the dry matter recovery were optimized by response surface methodology (RSM). The independent variables were coded as: pH (x1), incubation temperature (x2), the total content of combined cellulase-pectinase (x3) (with the ratio of cellulase/pectinase was 1/1), and hydrolysis time (x4). The results of the analysis of variance (ANOVA) showed that the variables actively affected the efficiency of extracted dry matter. The optimal conditions of hydrolysis were derived at Z1 = , Z2 = 45 oC, Z3 = % (v/dwt), Z4 = 120 minutes. At the conditions, the efficiency of the prediction model reached % and it had no significant difference compared with experimental value ( %). That increased % compared with the efficiency from the non-enzymatic extraction. Besides, the recovery efficiency of carbohydrate reached %. Further, the content of extracted phenolic, carotenoids and DPPH and ABTS radical scavenging activity .
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