tailieunhanh - Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia

Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. | H. TEYEB, N. ZANINA, M. NEFFATI, W. DOUKI, M. F. NAJJAR Turk J Biol 36 (2012) 53-58 © TÜBİTAK doi: Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia Hassen TEYEB1,2, Nahla ZANINA3, Mohamed NEFFATI2, Wahiba DOUKI1, Mohamed Fadhel NAJJAR1 1 Biochemistry and Toxicology Laboratory, University Hospital of Monastir, Monastir - TUNISIA 2 Range Ecology Laboratory, Arid Land Institute of Medenine, Medenine - TUNISIA 3 Biophysics Laboratory, Faculty of Medicine, Monastir University, Monastir - TUNISIA Received: Abstract: Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diffusion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at IC50 = 85 ± μg/mL. The best incubation time for this extract was 48 h. Most of the tested extracts were active against the tested bacterial strains. The methanol extract was found to be the most active against Pseudomonas aeruginosa and Salmonella typhimurium. This study is the first report on the biological activity of leaf extracts of Astragalus gombiformis with respect to antibacterial activity as well as cytotoxicity. Key words: Astragalus gombiformis, antibacterial effect, cytotoxicity Introduction In recent decades, many studies have been carried out on different plant species to discover compounds of possible interest for medicinal application against various diseases, such as cancer, oxidative stress, and fungal, .

• Sinh học
• Astragalus gombiformis
• Antibacterial effect
• Antibacterial activities
• Genus Astragalus
• Dissolved in dimethyl sulfoxide