tailieunhanh - Production of a novel bifunctional catalase-phenol oxidase of Scytalidium thermophilum in the presence of phenolic compounds
A novel bifunctional catalase with additional phenol oxidase activity (CATPO) is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. | Y. YÜZÜGÜLLÜ, Z. B. ÖGEL, BÖLÜKBAŞI, N. ÇORUH, G. KARAKAŞ Turk J Biol 35 (2011) 697-704 © TÜBİTAK doi: Production of a novel bifunctional catalase-phenol oxidase of Scytalidium thermophilum in the presence of phenolic compounds Yonca YÜZÜGÜLLÜ1, Zümrüt Begüm ÖGEL2, Ufuk BAKIR BÖLÜKBAŞI3, Nursen ÇORUH4, Gürkan KARAKAŞ3 1 Middle East Technical University, Faculty of Arts and Sciences, Biotechnology Graduate Program, 06531 Ankara - TURKEY 2 Middle East Technical University, Faculty of Engineering, Food Engineering Department, 06531 Ankara - TURKEY 3 Middle East Technical University, Faculty of Engineering, Chemical Engineering Department, 06531 Ankara - TURKEY 4 Middle East Technical University, Faculty of Arts and Sciences, Department of Chemistry, 06531 Ankara - TURKEY Received: Abstract: A novel bifunctional catalase with additional phenol oxidase activity (CATPO) is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. It was determined that some phenolics exerted a negative effect on growth (catechol, coumaric acid, hydroquinone, kaempferol, myricetin), while others either did not influence growth in a negative manner or enhanced growth by a maximum of 50% increase in biomass (caffeic acid, chlorogenic acid, catechin, gallic acid, resorcinol, vanillic acid). Hydroquinone and myricetin showed an antifungal effect and enhanced CATPO production, while catechol, coumaric acid, and kaempferol decreased CATPO production and showed a toxic effect at higher doses. In general, phenolics acting in an antioxidant manner exhibited a reverse interrelation with CATPO production. These results suggest that the presence of antioxidant phenolic compounds has an effect on enhancing its antioxidant activity; enzyme is .
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