tailieunhanh - Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. | S. ÜLKER, A. ÖZEL, A. ÇOLAK, Ş. ALPAY KARAOĞLU Turk J Biol 35 (2011) 543-550 © TÜBİTAK doi: Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil Serdar ÜLKER1, Arzu ÖZEL2, Ahmet ÇOLAK3, Şengül ALPAY KARAOĞLU1 1 Department of Biology, Faculty of Arts and Sciences, Rize University, Rize - TURKEY 2 Department of Chemistry, Faculty of Arts and Sciences, Rize University, Rize - TURKEY 3 Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, Trabzon - TURKEY Received: Abstract: This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were and 40 °C, respectively. The lipase was stable at a pH range of and at 40 °C for 60 min. Ca2+ and Mn2+ enhanced lipase activity but it was determined that other metallic ions did not affect the enzyme activity. The Km and Vmax values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be mM and mM/min, respectively. Key words: Trichoderma harzianum, extracellular lipase, enzyme characterization Topraktan izole edilen Trichoderma harzianum hücre dışı lipazının izolasyonu, üretimi ve karakterizasyonu Özet: Bu çalışma, Trichoderma harzianum lipazının karakterizasyonu hakkında ilk rapordur. Yeni bir suş olan Trichoderma harzianum IDM14D topraktan izole edildi. İzole edilen bu suş, lipaz üretimi için çalkalamalı kültürde 30 °C’de 7 gün inkübe edilmiştir. Lipaz üretimi için en iyi karbon kaynağı glukoz, en iyi azot kaynağının pepton .
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