tailieunhanh - Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw

The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol, chloroform, acetone, ethyl acetate A, B, C, and D) by two different extraction processes. | F. SAVAROĞLU, C. FİLİK İŞÇEN, A. P. ÖZTOPCU VATAN, S. KABADERE, S. İLHAN, R. UYAR Turk J Biol 35 (2011) 361-369 © TÜBİTAK doi: Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw. Filiz SAVAROĞLU1, Cansu FİLİK İŞÇEN2, Ayşe Pınar ÖZTOPCU VATAN1, Selda KABADERE3, Semra İLHAN1, Ruhi UYAR3 1 Department of Biology, Faculty of Art and Sciences, Eskişehir Osmangazi University, 26480 Eskişehir - TURKEY 2 Department of Elementary Education, Faculty of Education, Eskişehir Osmangazi University, 26480 Eskişehir - TURKEY 3 Department of Physiology, Faculty of Medicine, Eskişehir Osmangazi University, 26480 Eskişehir-TURKEY Received: Abstract: The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then screened through a preparative TLC (bioautography) for its active components. Spot 4 of extract C exhibited the highest antimicrobial activity against 5 bacterial strains (B. cereus, B. subtilis, E. faecalis, E. aerogenes, and Y. enterocolitica) and 3 fungal strains (A. flavus, F. solani, and F. graminearum), with MIC values of μg/mL and μg/mL, respectively. In addition, in vitro toxicity of the active component of extract C at the concentrations of , , 16, 80, and 160 μg/mL was tested. Depending on dose and time, only 80 and 160 μg/mL decreased rat glioma (C6) cell