tailieunhanh - Transcription of the Autographa californica nuclear polyhedrosis virus p39 gene in Bombyx mori cell culture

Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. | Tr. J. of Biology 22 (1998) 153-159 © TÜBİTAK Z. DEMİRBAĞ Transcription of the Autographa californica Nuclear Polyhedrosis Virus p39 Gene in Bombyx mori Cell Culture Zihni DEMİRBAĞ Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü,Trabzon-TURKEY Received: Abstract:Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. This is the first transcriptional study of this gene in the Bombyx mori cell line. In this study, comparative transcription of the AcNPV p39 gene was examined in S. frugiperda and B. mori cell cultures. Northern blot hybridization of totoal RNA samples, isolated from mock-infected (O and 48 hour post infection, .) and AcNPV-infected (0, 2, 6, 12, 24, 48 .) S. frugiperda and B. mori cell lines, was analyzed. Results indicates that the kb RNA fragment, specific to the p39 gene, is present in AcNPV-infected S. frugiperda and Bombyx mori cell lines. Moreover, the amount of transcript is higher and transcription occurs later in the cell line than in the S. frugiperda cell line. Key Words: AcNPV, Baculovirus, p39 gene, transcription, Bombyx mori Autographa californica Nüklear Polinedrozis Virüs’ün p39 Geninin Bombyx mori Hücre Kültüründe Transkripsiyonu Özet: Autographa californica nüklear polihedrozis virüs (AcNPV, Familya: Baculovirus)'ün p39 geninin (erken gen) transkripsiyonu, Spodoptera frugiperda üretken sisteminde yoğun bir şekilde çalışılmıştır. Bu, Bomyx mori hücre kültüründe yapılan p39 genine ait ilk transkripsiyonel calışmadır. Bu çalışmada, AcNPV’ p39 geninin S. frugiperda ve B. mori hücrelerindeki karşılaştırmalı transkripsiyonu araştırıldı. AcNPV ile enfekte olmamış (0 ve 48 saat sonra) ve enfekte olmuş (0, 2, 6, 12, 24 ve 48 saat enfeksiyon sonrası) S. frugiperda ve B. mori hücre suşlarından izole edilen toplam RNA .

• Sinh học
• Autographa californica nuclear polynedrosis virus
• Delayed early gene
• Spodoptera frugiperda cells
• S
• frugiperda cell line
• RNA fragment
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• BMC Genetics
• Transfer RNA
• tRNA derived fragment
• Deep sequencing analysis
• Tadpole shrimp
• BMC Genomics
• Degenerative joint disease
• Animal model
• Metacarpophalangeal joint
• Osteochondral fragment
• RNA seq