tailieunhanh - Determination of genetic relationships among Velezia L. (Caryophyllaceae) species using RAPD markers

Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identification. | İ. ERÖZ POYRAZ, E. SÖZEN, E. ATAŞLAR, İ. POYRAZ Turk J Biol 36 (2012) 293-302 © TÜBİTAK doi: Determination of genetic relationships among Velezia L. (Caryophyllaceae) species using RAPD markers İlham ERÖZ POYRAZ1, Emel SÖZEN2, Ebru ATAŞLAR3, İsmail POYRAZ4 1 Department of Pharmaceutical Botany, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir - TURKEY 2 Department of Biology, Faculty of Sciences, Anadolu University, 26470 Eskişehir - TURKEY 3 Department of Biology, Faculty of Science and Literature, Eskişehir Osmangazi University, 26480 Eskişehir - TURKEY 4 Department of Molecular Biology and Genetics, Faculty of Science and Letters, Bilecik University, 11210 Gülümbe, Bilecik - TURKEY Received: Abstract: Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high () and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identification. Furthermore, it can be efficiently employed in future studies to provide preliminary data for conservation of endangered Velezia species. Key words: Genetic relationships, RAPD-PCR, Velezia L., Turkey RAPD markörleri kullanılarak Velezia L. (Caryophyllaceae) türlerinin genetik akrabalıklarının belirlenmesi Özet: Bu çalışmada, RAPD (Random Amplified Polymorphic DNA) belirteçleri Türkiye’de yetişen Velezia L. .