tailieunhanh - Determination of genetic variation in populations of Bemisia tabaci in Antalya

Although a number of investigations on the molecular genetics of Bemisia tabaci (Gennadius) have appeared in the literature, no such studies are known from Turkey. | Turk J Agric For 26 (2002) 211-216 © TÜB‹TAK Determination of Genetic Variation in Populations of Bemisia tabaci in Antalya Hüseyin GÖÇMEN Akdeniz University, Faculty of Agriculture, Department of Plant Protection, Antalya - TURKEY Zübeyir DEVRAN Citrus and Greenhouse Research Institute, Antalya - TURKEY Received: Abstract: Although a number of investigations on the molecular genetics of Bemisia tabaci (Gennadius) have appeared in the literature, no such studies are known from Turkey. In order to fill this gap the amplified fragment length polymorphism (AFLP) technique was used to assay eight populations of B. tabaci collected from cotton, protected vegetables (tomato, cucumber and eggplant) and peppermint in various locations in the province of Antalya, Turkey. Eleven AFLP primer pairs detected a total of 547 AFLP fragments in these populations and 389 of these showed polymorphism. Cluster analyses of AFLP data clearly separated these populations into two groups with a genetic variability between 42% and 81%. The results indicated that B. tabaci may have biotypes based on adaptation to certain host plant species in Turkey. Key Words: Bemisia tabaci, AFLP, genetic variability, Antalya Antalya’da Bemisia tabaci Populasyonlar› Aras›ndaki Genetik Variyasyonun Saptanmas› Özet: Bemisia tabaci (Genn.) üzerinde bir çok ülkede molekuler genetik çal›flmalar yap›l›rken Türkiye’de bu tür bir çal›flma bilinmemektedir. Bu bofllugu doldurmak amac›yla Antalya’n›n degiflik yerlerinden pamuk, örtü alt›nda yetifltirilen sebzeler (domates, h›yar, patl›can) ve yabani nane üzerinden toplanan sekiz B. tabaci populasyonu aras›ndaki genetik varyasyonun araflt›r›lmas›nda AFLP (Amplified Fragment Length Polymorphism) teknigi kullan›lm›flt›r. Onbir AFLP primeri kulan›larak yap›lan çal›flmada 547 AFLP fragmenti saptanm›fl ve bunlardan 389’u polymorfizm göstermifltir. AFLP verilerinin cluster analizi sonucu bu populasyonlar %42 ile %81 aras›nda genetik benzerlik gösteren iki .

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