tailieunhanh - L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: Immunohistochemical and electron microscopic study
Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties, among them is the acceleration of wound healing, especially in the stomach. | Turkish Journal of Biology Turk J Biol (2013) 37: 342-349 © TÜBİTAK doi: Research Article L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: immunohistochemical and electron microscopic study 1,3, 1 Samah KANDEEL 1 1 1 *, Thoryaa EL-DEEB , Ehsan SALAH , Nafisa EL-BAKARY , Naglaa SARHAN , 2 3 3 Mohamed BALAHA , Hiroki SAKAI , Tokuma YANAI 1 Department of Histology, Faculty of Medicine, Tanta University, Tanta, Egypt 2 Department of Pharmacology, Faculty of Medicine, Tanta University, Tanta, Egypt 3 Department of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan Received: Accepted: Published Online: Printed: Abstract: Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine and mL/kg ethylene glycol for 2 weeks). By examination, group B showed significant increases in caspase-3 positive cells in comparison to the control, semithin sections revealed surface disruption and ulcerations, and ultrathin sections showed multiple pathological changes in cells lining the gastric mucosal glands. In group C significant decreases in caspase-3 positive cells were encountered and the other pathological findings in group B were found to be .
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