tailieunhanh - The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio)
This study investigated the developmental toxicity of zinc chloride (ZnCl2) in zebrafish embryos (Brachydanio rerio, Cyprinidae, Cypriniformes). Zebrafish embryos were exposed to 5 different concentrations of ZnCl2, from the blastula stage, for 15 days under static renewal test conditions. | Turkish Journal of Biology Research Article Turk J Biol (2013) 37: 158-164 © TÜBİTAK doi: The effects of zinc chloride during early embryonic development in zebrafish (Brachydanio rerio) 1 2 2, Melek KÜÇÜKOĞLU , Uğur Seçil BİNOKAY , Ayper BOĞA PEKMEZEKMEK * 1 Alumna Faculty Member, Çukurova University, Adana, Turkey 2 Department of Physiology, Faculty of Medicine, Çukurova University, Adana, Turkey Received: Accepted: Published Online: Printed: Abstract: This study investigated the developmental toxicity of zinc chloride (ZnCl2) in zebrafish embryos (Brachydanio rerio, Cyprinidae, Cypriniformes). Zebrafish embryos were exposed to 5 different concentrations of ZnCl2, from the blastula stage, for 15 days under static renewal test conditions. As a result, the corresponding median lethal concentration (LC50) value determined for ZnCl2 exposure was mg/L ( mg/L as a lone Zn2+ ion). At mg/L ZnCl2, the exposed group’s hatching began at 7 days instead of at 4 days, and most of the embryos died in the chorion without hatching at 11 and 12 days. Developmental deformities such as abnormal embryogenesis, low hatchability, delayed hatching, and reduction of newly hatched larvae, and a poor survival ratio (mortality ratio of and 10 mg/L concentrations compared to control, P ), while differences between both groups were significant at the concentration of 1 mg/L ZnCl2 (P < ). It was also found that when comparing the final mortality percentage at 1 mg/L with mg/L and with 10 mg/L ZnCl2 concentrations, the differences were significant (P < and P < , respectively). When investigating mortality and anomaly percentages of the final concentration of mg/L ZnCl2, significant differences were found, at 60% and , respectively. These differences could be attributed to deaths of embryos and larvae that showed no anomalies under .
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