tailieunhanh - Effects of α-lipoic acid on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in sub-chronic thinner-addicted rats

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant−antioxidant balance, the relation between toxicity and oxidative stress, and a possible protective effect of α-lipoic acid against thinner toxication in rats. | Turk J Biol 36 (2012) 702-710 © TÜBİTAK doi: Effects of α-lipoic acid on DNA damage, protein oxidation, lipid peroxidation, and some biochemical parameters in sub-chronic thinner-addicted rats 1 Muhsin KONUK1,*, Tuğba ŞAHİN2, İbrahim Hakkı CİĞERCİ2, 3 2 Abdurrahman Fatih FİDAN , Safiye Elif KORCAN Molecular Biology and Genetics Department, Faculty of Engineering and Natural Sciences, Üsküdar University, Altunizade, 34662 İstanbul − TURKEY 2 3 Biology Department, Faculty of Science and Literature, Afyon Kocatepe University, 03200 Afyonkarahisar − TURKEY Biochemistry Department, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar − TURKEY Received: ● Accepted: Abstract: The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant− antioxidant balance, the relation between toxicity and oxidative stress, and a possible protective effect of α-lipoic acid against thinner toxication in rats. Sprague−Dawley rats were divided into 5 groups as follows: control (K), olive oil (Z), α-lipoic acid (L), thinner (T), and α-lipoic acid + thinner (LAT). Each group was composed of 15 rats, and the study lasted 8 weeks. After completing the animal studies malondialdehyde (MDA), reduced glutathione (GSH), methemoglobin (MetHb), toluol, and mononuclear leucocyte damage levels; protein oxidation, nitric oxide (NOx) metabolites, total antioxidant capacity (TAC), glucose, total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined from blood specimens of the rats. The data obtained from the study were statistically analyzed using SPSS, and both ANOVA and Duncan tests were performed. P 13) at 4 °C for unwinding (40 min). All steps were carried out under minimal illumination. After electrophoresis the slides were neutralized ( M Tris-HCl, pH ) for 5 min. The dried