tailieunhanh - Antioxidant enzymes and GST activity in natural populations of Holandriana holandrii from the Bosna River
Specimens of the freshwater snail Holandriana holandrii affected by different levels of contamination were collected from 3 sites within the Bosna River Basin, . Visoko, Doboj, and Modrica. | Turk J Biol 36 (2012) 477-485 © TÜBİTAK doi: Antioxidant enzymes and GST activity in natural populations of Holandriana holandrii from the Bosna River 1 2 3 4 Jelena VRANKOVIĆ , Svetlana LABUS-BLAGOJEVIĆ , Béla CSANYI , Jarmila MAKOVINSKA , 5 6 1 1 Olga CVETKOVIĆ , Zoran GAČIĆ , Duško BLAGOJEVIĆ , Momir PAUNOVIĆ 1 Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11060 Belgrade - SERBIA 2 Institute of Public Health of Serbia “Dr Milan Jovanović Batut”, 11000 Belgrade - SERBIA 3 VITUKI Kht., 1095 Budapest - HUNGARY 4 Water Research Institute, 812 49 Bratislava - SLOVAK REPUBLIC 5 Institute of Chemistry, Technology and Metallurgy, Center of Chemistry, University of Belgrade,11000 Belgrade - SERBIA 6 Institute for Multidisciplinary Research, University of Belgrade, 11060 Belgrade - SERBIA Received: ● Accepted: Abstract: Specimens of the freshwater snail Holandriana holandrii affected by different levels of contamination were collected from 3 sites within the Bosna River Basin, . Visoko, Doboj, and Modrica. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) were investigated in order to understand their variation with respect to the pollution status of the sampling locations. The results revealed a significant increase in CAT, GPx, and GST activities in the snails collected from Modrica, suggesting that the animals at this location are exposed to a higher level of oxidative stress as compared to those from Visoko and Doboj. On the other hand, increased SOD activity measured in specimens from Visoko was indicative of the presence of increased levels of superoxide anion radical. No snails from any location were significantly exposed to organic pollution, since its concentration in the whole body homogenates was below the limit of detection. Our findings
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