tailieunhanh - Cloning and sequencing of coat protein gene of Zucchini yellow mosaic virus isolated from squash and muskmelon in Turkey

The coat protein (CP) genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) and ZYMV-Ahlat (Ah), respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined. | Turk J Biol 36 (2012) 423-429 © TÜBİTAK doi: Cloning and sequencing of coat protein gene of Zucchini yellow mosaic virus isolated from squash and muskmelon in Turkey Meryem ÖZER1, Hikmet Murat SİPAHİOĞLU1, Mustafa USTA1, Hakan FİDAN2 1 Department of Plant Protection, Faculty of Agriculture, Yüzüncü Yıl University, 65080 Van - TURKEY 2 Biological Control Research Station, 01321 Yüreğir Adana - TURKEY Received: ● Accepted: Abstract: The coat protein (CP) genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) and ZYMV-Ahlat (Ah), respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined. The analysis revealed that both Turkish ZYMV-CP genes contained 837 nucleotides encoded for a CP of about kDa. Phylogenetic trees based on nucleic acid sequences were constructed by the neighbor joining and unweighted pair group mean arithmetic (UPGMA) methods with 100 bootstrap replicates. A high degree of homology was detected between the 2 Turkish isolates on the nucleotide sequences (97%). The CP sequence of ZYMV-Ad and ZYMV-Ah varied among the 23 isolates with overall identity of 93%-98% and 94%-99%, respectively, at the nucleotide level. Comparison of the nucleotide sequences of 23 isolates from different geographical regions worldwide showed the ZYMV-Ad isolate clustered with isolates from Middle Eastern countries (Israel, Jordan, and Syria); ZYMV-Ah isolate was clustered with isolates from Far Eastern countries (Korea and Taiwan). The N-terminal of the Turkish ZYMV-Ah CP contained a distinctive sequence at nucleotide positions 93249328, which distinguished the Turkish ZYMV-Ah isolate from all previously reported ZYMV isolates. The CP cistron of the ZYMV-Ad and Ah isolates contained 279 amino acid residues. Pairwise nucleotide sequence comparison revealed sequence similarities of 58%-70% between .

• Sinh học
• Coat protein
• Phylogenetic analysis
• Coat protein gene
• Genomic RNA
• Zucchini yellow mosaic virus
• Recombinant coat protein
• TA cloning
• His tag purification
• Cloning and expression
• Tomato leaf curl virus
• Sap transmission
• Gemini virus
• AV1 coat protein gene
• Chilli leaf curl virus
• Banana bract mosaic virus
• Mysore poovan
• Molecular cloning and characterization
• Yellow mosaic virus
• Sequence comparision
• Horse gram yellow mosaic Virus
• Blackgram yellow mosaic virus
• Nucleotide sequence identity
• Amino acid identity
• BMC Plant Biology
• DELLA proteins
• SA signaling
• Defense response
• CPV 2b and PDB
• B Cell linear epitope prediction
• VP2 partial coat protein predicted model
• CPV 2b isolate using bioinformatics
• CPV 2b isolate
• Southern blot
• Expression vector
• Banana bunchy top viral coat protein
• Gene expression studies
• Molecular level
• Cucumber mosaic virus
• Infectious chlorosis
• Molecular characterization
• Complete coat protein gene sequence
• CMV infecting banana
• Scientific reports
• breast cancer
• medicine
• Sesbania
• chemical reaction
• tumor cells
• báo cáo khoa học
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• cách trình bày báo cáo
• thesis report
• medical report
• molecular Biology
• Antimicrobial
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• Report medicine
• traditional medicine
• genetics
• Structural biochemistry
• Structural Biology
• biochemical studies
• environmental medicine
• Crystal structure
• medical knowledge
• medical research
• analysis of cytoplasmic
• Tạp chí khoa học
• Tạp chí sinh học
• Công nghệ sinh học
• Chuyển gen kháng virus
• Virus gây bệnh khảm lá
• Bệnh ở cây trồng
• Chuyển gen kháng bệnh cho đậu
• Citrus tristeza virus
• Coat protein genes
• Colony PCR hybridization
• Direct PCR hybridization
• Double stranded RNA
• Replicase and RNA mediated resistance
• Plant transformation
• RNA dependent RNA polymerase
• Whiteflies and phylogenetic relationship
• Phylogenetic relationship
• Tomato leaf curl virus associated
• Silico characterization
• LAMP assay
• Ridge gourd
• Yellow mosaic disease
• Ridge gourd [Luffa acutangula (L) Roxb
• Loop mediated isothermal amplification assay
• Mungbean yellow mosaic virus
• Mungbean yellow mosaic India virus