tailieunhanh - Development of the downy mildew pathogen Bremia lactucae on transgenic lettuce expressing a bacterial ß 1,3 glucanase

A β-1,3-glucanase gene from Arthrobacter sp. driven by the 35S promoter was singly transformed into two lettuce cultivars, Cobham Gree and Diana, using the binary vector system of Agrobacterium tumefaciens. | Tr. J. of Agriculture and Forestry 22 (1998) 313-321 © TÜBİTAK Y. DEDE Development of the Downy Mildew Pathogen Bremia Lactucae on Transgenic Lettuce Expressing a Bacterial ß-1,3-Glucanase Yeter DEDE Çukurova Üniversitesi Ziraat Fakültesi, Bitki Koruma Bölümü, Adana-TÜRKİYE Received: Abstract: A β-1,3-glucanase gene from Arthrobacter sp. driven by the 35S promoter was singly transformed into two lettuce cultivars, Cobham Gree and Diana, using the binary vector system of Agrobacterium tumefaciens. Tansformation was confirmed by using Southern and Northern analysis, Npt II enzyme assays and segregation of resistance to kanamycin. Transgenic plants were infected with the letuce downy mildew fungus, Bremia lactucae which contains β-1,3-gluncan in its cell wall and alternations in the development the fungus could easily be monitored. Transgenic plants inoculated with B. lactucae showed different levels of resistant responses compared to the control and they were examined microscopically. Bakteriyel bir ß-1,3-Glukanaz Geni Transfer Edilen Marul Bitkisinde Mildiyö Patojeni Bremia Lactucae’ nın Gelişimi Özet: Marul çeşitleri Diana ve Cobham Creen’e Agrobacterium tumefaciens’in bitkilere gen transfer etme özelliğinden faydalanarak Archrobacter sp. orjinli bir β-1,3-glukanaz geni 35S promoter kontrolünde transfer edilmiştir. Gen transferi Southern ve Northern hibridizasyon teknikleriyle, NPT II enzim aktivite testi ve kanamisine dayanıklıktaki genetik açılma ile kanıtlanmıştır. Gen transferi yapılan bitkiler hücre duvarında β-1,3-glukan içeren ve gelişimi kolayca izlenebilen mildyö etmeni Bremia lactucae ile infekte edilmiştir. Kontrole oranla bu bitkilerde değişik seviyelerde dayanıklık reaksiyonları gözlenmiş ve bunlar mikroskobik olarak incelenmiştir. Introduction Induced resistance in many plants is accompanied by the induced synthesis of PR (Pathagenesis-Related) proteins including chitinase and β-1,3-glucanase. β1,3-glucanases are involved in the .