tailieunhanh - Rapid characterization of garlic clones with locus specific DNA markers

Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. | Turk J Agric For 32 (2008) 357-362 © TÜB‹TAK Rapid Characterization of Garlic Clones with Locus-Specific DNA Markers Meryem ‹PEK1,*, Ahmet ‹PEK1, Philipp W. SIMON2 1 Uluda¤ University, Faculty of Agriculture, Horticulture Department, Görükle 16059 Bursa - TURKEY 2 University of Wisconsin, Department of Horticulture and . Department of Agriculture-Agricultural Research Service Vegetable Crops Research Unit, 1575 Linden Drive, Madison, WI 53706, USA Received: Abstract: Maintenance of redundant garlic (Allium sativum L.) accessions is expensive due to the necessity of yearly regenerating garlic accessions in germplasm centers. Therefore, rapid characterization of garlic accessions is important for avoiding duplicated genotypes. For this purpose we developed several locus-specific polymerase chain reaction (PCR)-based DNA markers, and tested them for the characterization of garlic clones that were previously analyzed using amplified fragment length polymorphism (AFLP) and randomly amplified polymorphic DNA (RAPD) markers. Among 25 garlic clones, 4 locus-specific markers generated 24 polymorphic alleles. Garlic accessions within a group determined by AFLP and RAPD analyses generally had the same alleles as the locus-specific markers. In addition, several alleles of these locus-specific markers were associated only with certain AFLP and RAPD groups, which can allow rapid assessment of newly collected garlic accessions; therefore, these locus-specific markers can be used as another tool for the rapid characterization of garlic germplasm collections. Key Words: Allium sativum L., genetic characterization, DNA markers, germplasm Sar›msak Klonlar›n›n Lokus Spesifik DNA Mark›rlar›yla H›zl› Tan›mlanmas› Özet: Gen bankalar›nda sar›msak klonlar›n› muhafaza etmek için her y›l yeniden üretilmesi gerekti¤inden ayn› genotipe sahip sar›msak klonlar›n› gen bankalar›nda muhafaza etmek pahal›d›r. Bu nedenle gen bankalar›nda ayn› genotipe sahip sar›msak klonlar›n›n .

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