tailieunhanh - High efficiency somatic embryogenesis from immature zygotic embryos of grapevine: The effect of genotype, media, 2,4-D, and incubation conditions
Immature zygotic embryos of 41 B grape rootstock (V. vinifera L. cv. ‘Chasselas’ × V. berlandieri) and Yalova ‹ncisi (V. vinifera L.) were cultured on MS, NN, and B5 media supplemented with , 1, 2, and 4 mg l-1 2,4-D at 16/8 h light/dark photoperiod and complete darkness. | Turk J Agric For 32 (2008) 311-317 © TÜB‹TAK High Efficiency Somatic Embryogenesis from Immature Zygotic Embryos of Grapevine: The Effect of Genotype, Media, 2,4-D, and Incubation Conditions Serpil GÖK TANGOLAR1,*, Saadet BÜYÜKALACA2, Fuat ERGENO⁄LU2 1 Plant Protection Research Institute, Adana - TURKEY 2University of Çukurova, Faculty of Agriculture, Department of Horticulture, Adana - TURKEY Received: Abstract: Immature zygotic embryos of 41 B grape rootstock (V. vinifera L. cv. ‘Chasselas’ × V. berlandieri) and Yalova ‹ncisi (V. vinifera L.) were cultured on MS, NN, and B5 media supplemented with , 1, 2, and 4 mg l-1 2,4-D at 16/8 h light/dark photoperiod and complete darkness. For 41 B rootstock, the highest somatic embryo formation was obtained from dark condition on B5 media containing and 1 mg l-1 2,4-D with the rates of 30% and , respectively. Yalova ‹ncisi zygotic embryos produced 5% somatic embryos only at 16/8 h light/dark photoperiod of MS supplemented with 1 mg l-1 2,4-D, and also in 16/8 h light/dark photoperiod and complete darkness somatic embryos were produced at the rates of and , respectively, in cultures of NN containing mg l-1 2,4-D. After 8 months of culture, 559 embryos at torpedo stage were identified on B5 + 1 mg l-1 2,4-D + dark cultures of 41 B and 912 embryos at torpedo stage were identified on NN + mg l-1 2,4-D + light cultures of Yalova ‹ncisi. The highest germination and plantlet conversion rates were obtained from 41 B on free NN medium (58% and 75%, respectively) and from Yalova ‹ncisi on free MS medium ( and 45%, respectively). Of the regenerated somatic plantlets, were successfully transferred to soil. Key Words: Grapevine, zygotic embryo, somatic embryo, regeneration Asmada Olgunlaflmam›fl Zigotik Embriyolardan Yüksek Oranda Somatik Embriyo Oluflumu: Genotip, Besi Ortam›, 2,4-D ve ‹nkübasyon Koflullar›n›n Etkisi Özet: 41 B Amerikan asma anac› (V. vinifera L. cv. ‘Chasselas’ ×
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