tailieunhanh - Chemical examination of Hydrocotyle bonariensis L. (Apiaceae)

The major components of stem-leaf are: (Z)-3-hexen-1-ol, trans-caryophyllene, αfarnesene, copaene while the major components of flower are: α-pinene, 2-β-pinene, β-myrcene, limonene, α-caryophyllene, epibicyclosesquiphellandrene. The essential oil of the stem-leaf showed cytotoxic activities in vitro on RD and Hep-G2 cancer cells with the IC50 values of and , respectively. The study on the plant is being continued. | Journal of Chemistry, Vol. 47 (5), P. 542 - 546, 2009 CHEMICAL EXAMINATION OF HYDROCOTYLE BONARIENSIS L. (APIACEAE) Received 19 Oct 2007 1 TON NU LIEN HUONG , NGUYEN KIM PHI PHUNG2, NGUYEN NGOC SUONG2 1 Department of Sciences, University of Can Tho 2 University of Natural Sciences, National University Ho Chi Minh City Abstract Hydrocotyle bonariensis, growing in the Mekong-delta, is used as vegetable and has not yet been chemically studied. From the aerial parts of H. bonariensis, three compounds had been isolated: a triterpene squalene (1) and a mixture of two lignans: hinokinin (2) and α-[3,4methylendioxy phenylmethylidene]-β-[3,4-methylendioxyphenylmethyl]-γ-butyrolactone (3). The fresh plant was divided into stem-leaf and flower and each part was distilled with steam to afford essential oils. The major components of stem-leaf are: (Z)-3-hexen-1-ol, trans-caryophyllene, αfarnesene, copaene while the major components of flower are: α-pinene, 2-β-pinene, β-myrcene, limonene, α-caryophyllene, epibicyclosesquiphellandrene. The essential oil of the stem-leaf showed cytotoxic activities in vitro on RD and Hep-G2 cancer cells with the IC50 values of and , respectively. The study on the plant is being continued. 3 1 5 7 9 11 2 20 O 30 O 16 18 14 19 O 6 15 17 29 3 13 12 11 4 2 9 8 βα O 1 O 7 28 (1) Squalene 10 O 20a O O O 16a 17a 14a 13a 3a 4a 6a 7a 10a O 12a (2) Hinokinin 18a β α 2a 9a 8a 11a [α,β- bis(3,4-Methylendioxyphenylmethyl)O 1a O (3) -γ-butyrolactone] α-(3,4-Methylendioxyphenylmethyliden)-β-(3,4-methylendioxyphenylmethyl)-γ-butyrolactone Hydrocotyle bonariensis 1 - INTRODUCTION Hydrocotyle bonariensis, growing in the Mekong –delta, like H. asiatica and H. sibthorpioides, is used as vegetable. H. sibthorpioides Lam and H. maritima Honda showed hemostatic and antitumor activity [1]. Whole plant of Hydrocotyle and Centella 542 species (Apiaceae) produce characteristic essential oils. The major component