tailieunhanh - Báo cáo " ảnh hưởng một số điều kiện nuôi cấy chủng Asspergillus awamori VTCC-F-009 sinh tổng hợp Endo-B-1,4 Glucanase "

ảnh hưởng một số điều kiện nuôi cấy chủng Asspergillus awamori VTCC-F-009 sinh tổng hợp Endo-B-1,4 Glucanase | Tạp chi Công nghệ Sinh học 7 4 521-528 2009 EFFECTS OF CULTURE CONDITIONS ON THE PRODUCTION OF ENDO-P-1 4-GLUCANASE BY ASPERGILLUS AWAMOR1VTCC-F-099 Nguyen Van Tuan Quyen Dinh Thi Institute of Biotechnology SUMMARY Cellulases are a group of hydrolytic enzymes and are capable of degrading lignocellulosic materials. These enzymes are produced by several microorganisms including bacteria and fungi. Endo p-l 4-glucanase is one of three types cellulase which has a wide range of applications such as in food animal feed pulp industry waste management etc. Aspergillus awamori VTCC-F-099 A. awamori VTCC-F-099 strain produced the highest amount of endo p-l 4-glucanase CMCase among 26 A. awamori strains were screened. Some culture conditions for A. awamori VTCC-F-099 strain producing CMCase were examined to assess their effect on enzyme production. CMCase production of the strain A. awamori VTCC-F-099 was the highest after 96 hours of fermentation in MT1 medium with 2 CMC. The optimum temperature and pH for CMCase production from the strain A. awamori VTCC-F-099 were 30 C and respectively. The strain A. awamori VTCC-F-099 produced CMCase highest in case the corncob used as carbon source among tested carbon sources lactose glucose saccharose sugar-cane bagasse coffee grounds rice brand corncob peanut shell dried mandarin shell saw dust coconut fiber . Ammonium acetate was the best nitrogen source for the strain A. awamori VTCC-F-099 producing highest CMCase among tested nitrogen sources peptone urea fish powder soybean powder casein CH3COONH4 NH4NO3 NH SOẠ Keywords Aspergillus awamori VTCC-F-099 CMCase INTRODUCTION Cellulose is a major polysaccharide constituent of plant cell walls and one of the most abundant organic compounds in the biosphere Hong et al. 2001 Murai et al. 1998 . It is an unbranched glucose polymer composed of an p-1 4 glucose units linked by a P-l 4-D-glycosidic bond Gielkens et al. 1999 . Cellulose is commonly degraded by an enzyme complex .