tailieunhanh - Báo cáo "Một số yếu tố ảnh hưởng đến sinh khối cuả cây sâm Ngọc Linh (Panax Vietnamensis Ha et Grushv.) nuôi cấy In Vitro và bước đầu phân tích hàm lượng Saponin "

Một số yếu tố ảnh hưởng đến sinh khối cuả cây sâm Ngọc Linh (Panax Vietnamensis Ha et Grushv.) nuôi cấy In Vitro và bước đầu phân tích hàm lượng Saponin | Tạp chí Công nghệ Sinh học 7 3 357-370 2009 THE EFFECTS OF SOME FACTORS ON IN VITRO BIOMASS PRODUCTION OF VIETNAMESE GINSENG 7MAC4X VIETNAMENSIS HA ET GRUSHV. AND PRELIMINARY ANALYSIS OF SAPONIN CONTENT Duong Tan Nhut1 Vu Quoc Luan1 Nguyen Van Binh1 Pham Thanh Phong1 Bui Ngoc Huy1 Dang Thi Ngoc Ha1 Phan Quoc Tam1 Nguyen Ba Nam1 Vu Thi Hien1 Bui The Vinh2 Lam Thi My Hang1 Duong Thi Mong Ngoc2 Lam Bich Thao2 Tran Cong Luan2 Tay Nguyen Institute of Biology 2Research Center of Ginseng and Medicinal Materials - Hochiminh City SUMMARY Panax vietnamensis Ha et Grushv. a rare Panax genus of Vietnam is a well known Vietnamese ginseng Ngoe Linh Ginseng for its rich pharmaceutical compositions most importantly saponin. In order to obtain a stable and saponin-rich biomass of p. vietnamensis a tissue culture procedure was established. A TLC analysis of saponin composition was also conducted to investigate the presence of saponin in callus shoot and root biomass. Successful callus induction from leaf and petiole explants was obtained from MS medium supplemented with mg 1 2 4-D 2 4-dichlorophenoxyacetic acid mg 1 TDZ Thidiazuron under a photoperiod of 16 h. In the following steps the optimal auxin and its concentration appropriate photoperiod condition as well as callus size that were the best for callus proliferation were investigated. Among the auxins including 2 4-D IBA Indole-3-butyric acid and NAA a-Naphthaleneacetic acid 2 4-D at mg 1 was found to be the most effective for callus growth. Callus at the size of X cm grew the best as compared to bigger ones such as X cm and X cm. The effects of phytohormones sucrose and activated charcoal AC on shoot regeneration from callus and shoot proliferation have also been studied. Calli cultured on MS medium supplemented with mg 1 BA and mg 1 NAA regenerated more shoots. The suitable medium for shoot proliferation was MS Vi medium supplemented with mg 1 BA mg 1 NAA 50 g 1 sucrose and