tailieunhanh - Báo cáo: Đa dạng di truyền các giống xoài (mangifera sp.) bằng kỹ thuật sinh học phân tử

Ba mươi sáu giống xoài được thu thập trong một số tỉnh của Việt Nam, phần lớn tập trung ở tỉnh Đồng Tháp được phân tích đa dạng về di truyền thông qua việc sử dụng kỹ thuật Amplified Fragments Length Polymorphism (AFLP) và kỹ thuật giải trình tự dựa trên đoạn gen ITS (Internal Transcrip Space). | Tạp chí Khoa học 2012 22a 175-185 Trường Đại học Cần Thơ ĐA DẠNG DI TRUYỀN CÁC GIỐNG XOÀI MANGIFERA SP. BẰNG KỸ THUẬT SINH HỌC PHÂN TỬ Trần Nhân Dũng1 và Đỗ Tấn Khang1 ABSTRACT Genetic diversity of thirty six mango cultivars collected from some regions of Vietnam mostly in Dong Thap area were analyzed using combination of Amplified fragments length polymorphism AFLP technique and sequencing the ITS Internal Transcript Space region. The results showed that there were 149 peaks of AFLP observed and 49 peaks presented in all samples with the highest frequency. The genetical similarity was high with r 0 853. There were 2934 phylogenetic trees in ITS analysis. The final tree had the CI and it means the DNA sequences had a considerable difference. Based on results of both AFLP and ITS analysis two cultivars including Da and Gao mango cultivars were the same spieces. The Bom and Kensington Pride mango cultivars might origined from the same species. The Thuy Trieu mango cultivar and the cultivars planted in Nha Trang area with the similar phenotype derived from the same species with Thanh Ca mango cultivar grown in the South of Vietnam. The Bac-Tam-Bang variety which is Cambodian s favourite was a type of Hon xanh cultivar. The Cat Chu mango cultivars had a variety of genotype and phenotype. Thanh ca mango variety could be the original species of Tuong Thom and Cat Chu mango cultivars. Some of Thai mangoes had different genotype as well as phenotype with Vietnamese mangoes but the Manduongcao cultivar probably had a certain gene that is similar to Tuong cultivar. The Yen Chau mango cultivar in the Northern West of Vietnam had the same origin with the Kensington Pride and Bom cultivars which derived from Malaysia and Oceania respectively. Keywords AFLP molecular marker genetic diversity ITS mango cultivar Title Genetic diversity of mango Mangifera sp. cultivars using molecular biological techniques TÓM TẮT Ba mươi sáu giống xoài được thu thập trong một số tỉnh