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Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation

In recent years there has been a renewed interest in solid-state fermentation (SSF) processes for the production of bioactive compounds. While efforts continue largely to exploit filamentous fungi and yeasts for the production of various enzymes, attempts have also been made to explore possibilities of using bacterial strains in SSF systems (Pandey et al., 2000). Enzyme production by SSF using bacterial spp. has been reported for many enzymes such as xylanase (Gessesse and Mamo, 1999) and amylase (Babu and Satyanarayana, 1995) but reports on pectinase production by SSF using bacterial spp. are lacking in the literature. The use of SSF for the production of enzymes mainly from fungi may be due to the. | Available online at www.sciencedirect.com blOPEOPCi TfCfflOLOaY Bioresource Technology 88 2003 251-254 Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation Des Raj Kashyap a Sanjeev Kumar Soni a Rupinder Tewari b a Department of Microbiology Panjab University Chandigarh 160014 India b Department of Biotechnology Panjab University Chandigarh 160014 India Received 22 May 2002 received in revised form 10 August 2002 accepted 14 August 2002 Abstract Bacillus sp. DT7 produced very high levels of alkaline and thermotolerant pectinase by solid state fermentation. Production of this enzyme was affected by nature of solid substrate level of moisture content presence or absence of carbon nitrogen mineral and vitamin supplements. Maximum enzyme production of 8050 U g dry substrate was obtained in wheat bran supplemented with polygalacturonic acid PGA 1 w v and neurobion a multivitamin additive 27 l g dry substrate with distilled water at 75 moisture level after 36 h of incubation at 37 C. 2002 Published by Elsevier Science Ltd. Keywords Solid state fermentation Bacillus Pectinase 1. Introduction In recent years there has been a renewed interest in solid-state fermentation SSF processes for the production of bioactive compounds. While efforts continue largely to exploit filamentous fungi and yeasts for the production of various enzymes attempts have also been made to explore possibilities of using bacterial strains in SSF systems Pandey et al. 2000 . Enzyme production by SSF using bacterial spp. has been reported for many enzymes such as xylanase Gessesse and Mamo 1999 and amylase Babu and Satyanarayana 1995 but reports on pectinase production by SSF using bacterial spp. are lacking in the literature. The use of SSF for the production of enzymes mainly from fungi may be due to the general belief that SSF technique is applicable only to filamentous fungi Lonesane and Ghidyal 1992 . Studies on comparative production of pectinase by Aspergillus sp.