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Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix

Acharan sulfate, a recently discovered glycosaminoglycan isolated from Achatina fulica, has a major disaccharide repeating unit of fi4)-2-acetyl,2-deoxy-a-D-glucopyra-nose(1fi4)-2-sulfo-a-L-idopyranosyluronic acid (1fi,mak-ing it structurally related to both heparin and heparan sulfate. It has been suggested that this glycosaminoglycan is polydisperse, with an average molecular mass of 29 kDa andknownminor disaccharide sequence variants containing unsulfated iduronic acid. | Eur. J. Biochem. 271 845-854 2004 FEBS 2004 doi 10.1111 j.1432-1033.2004.03989.x Acharan sulfate the new glycosaminoglycan from Achatina fulica Bowdich 1822 Structural heterogeneity metabolic labeling and localization in the body mucus and the organic shell matrix Tuane C R. G. Vieira1 2 Adilson Costa-Filho1 2 Norma C Salaado3 Silvana Allodi4 Ana-Paula Valente2 5 . . . . Luiz E. Nasciutti4 and Luiz-Claudio F. Silva1 2 1Laboratorio de Tecido Conjuntivo Hospital Universitário Clementino Fraga Filho 2Departamento de Bioquímica Medica 3Laboratorio de Malacologia Museu Nacional 4Departamento de Histologia e Embriologia and 5Centro Nacional de Ressonancia Magnetica Nuclear de Macromoleculas Universidade Federal do Rio de Janeiro Brazil Acharan sulfate a recently discovered glycosaminoglycan isolated from Achatina fulica has a major disaccharide repeating unit of fi4 -2-acetyl 2-deoxy-a-D-glucopyra-nose 1fi4 -2-sulfo-a-L-idopyranosyluronic acid 1fi making it structurally related to both heparin and heparan sulfate. It has been suggested that this glycosaminoglycan is polydisperse with an average molecular mass of 29 kDa and known minor disaccharide sequence variants containing unsulfated iduronic acid. Acharan sulfate was found to be located in the body of this species using alcian blue staining and it was suggested to be the main constituent of the mucus. In the present work we provide further information on the structure and compartmental distribution of acharan sulfate in the snail body. Different populations of acharan sulfate presenting charge and or molecular mass heterogeneities were isolated from the whole body as well as from mucus and from the organic shell matrix. A minor glycosaminoglycan fraction susceptible to degradation by nitrous acid was also purified from the snail body suggesting the presence of N-sulfated glycosaminoglycan molecules. In addition we demonstrate the in vivo metabolic labeling of acharan sulfate in the snail body after a meal .