Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học: Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences

A phylogenetic analysis of more than 350 multicopper oxidases (MCOs) from fungi, insects, plants, and bacteria provided the basis for a refined classification of this enzyme family into laccasessensu stricto (basidiomyc-etous and ascomycetous), insect laccases, fungal pigment MCOs, fungal ferroxidases, ascorbate oxidases, plant laccase-like MCOs, and bilirubin oxidases. | iFEBS Journal Phylogenetic comparison and classification of laccase and related multicopper oxidase protein sequences Patrik J. Hoegger1 Sreedhar Kilaru1 Timothy Y. James2 Jason R. Thacker2 and Ursula Kues1 1 Georg-August-University Gottingen Institute of Forest Botany Gottingen Germany 2 Duke University Department of Biology Durham NC USA Keywords basidiomycetes evolution phylogeny wood decay white rot Correspondence P. J. Hoegger Georg-August-University Gottingen Institute of Forest Botany Buesgenweg 2 37077 Gottingen Germany Fax 49 551392705 Tel 49 5513914086 E-mail phoegge@gwdg.de Website http wwwuser.gwdg.de uffb mhb Database Protein sequence alignments are available in the EMBL-ALIGN database under the accession numbers ALIGN_000939 and ALIGN_000940 Received 24 October 2005 revised 17 March 2006 accepted 23 March 2006 doi 10.1111 j.1742-4658.2006.05247.x A phylogenetic analysis of more than 350 multicopper oxidases MCOs from fungi insects plants and bacteria provided the basis for a refined classification of this enzyme family into laccases sensu stricto basidiomyc-etous and ascomycetous insect laccases fungal pigment MCOs fungal ferroxidases ascorbate oxidases plant laccase-like MCOs and bilirubin oxidases. Within the largest group of enzymes formed by the 125 basidi-omycetous laccases the gene phylogeny does not strictly follow the species phylogeny. The enzymes seem to group at least partially according to the lifestyle of the corresponding species. Analyses of the completely sequenced fungal genomes showed that the composition of MCOs in the different species can be very variable. Some species seem to encode only ferroxidases whereas others have proteins which are distributed over up to four different functional clusters in the phylogenetic tree. Multicopper oxidases MCOs are a family of enzymes comprising laccases EC 1.10.3.2 ferroxidases EC 1.16.3.1 ascorbate oxidase EC 1.10.3.3 and ceruloplasmin. This family in turn belongs to the highly diverse group