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Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors
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We used a proteomic approach to identify constitutively formed extracellu-lar proteins of Hypocrea atroviridis(Trichoderma atroviride), a known bio-control agent. The fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis. | ễFEBS Journal Epl1 the major secreted protein of Hypocrea atroviridis on glucose is a member of a strongly conserved protein family comprising plant defense response elicitors Verena Seidl1 Martina Marchetti2 Reingard Schandl1 2 Gunter Allmaier2 and Christian P. Kubicek1 1 Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Austria 2 Institute of ChemicalTechnologies and Analytics Vienna University of Technology Austria Keywords cerato-platanin elicitor Hypocrea Trichoderma plant defense responses Correspondence V. Seidl Research Area Gene Technology and Applied Biochemistry Institute of ChemicalEngineering Vienna University of Technology Getreidemarkt 9 166-5 A-1060 Vienna Austria Fax 43 1 58801 17299 Tel 43 1 58801 17227 E-mail vseidl@mail.zserv.tuwien.ac.at Website http www.vt.tuwien.ac.at Received 30 March 2006 revised 25 July 2006 accepted 27 July 2006 doi 10.1111 j.1742-4658.2006.05435.x We used a proteomic approach to identify constitutively formed extracellular proteins of Hypocrea atroviridis Trichoderma atroviride a known biocontrol agent. The fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis. The two predominant spots were identified by MALDI MS utilizing peptide mass fingerprints and amino acid sequence tags obtained by postsource decay and or high-energy collision-induced dissociation MS MS experiments and turned out to be the same protein 12 629 Da as determined with MS pl 5.5-5.7 probably representing the monomer and the dimer. The corresponding gene was subsequently cloned from H. atroviridis and named epll eliciting plant response-like because it encodes a protein that exhibits high similarity to the cerato-platanin family which comprises proteins such as cerato-plata-nin from Ceratocystis fimbriata f. sp. platani and Snodprotl of Phaeos-phaeria nodorum which have been reported to be involved in plant pathogenesis and elicitation