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Burns Regenerative Medicine and Therapy - part 6

Khảo nghiệm chức năng miễn dịch tế bào. Các mẫu máu được lấy từ đuôi con chuột trong MEBO và nhóm kiểm soát, tương ứng, sau đó bôi và nhuộm màu theo các esterase acetate · naphthalene (ANAE) phương pháp. Những sau đó được kiểm tra dưới kính hiển vi. 100 tế bào lympho được quan sát ngẫu nhiên. | Fig. 14. a Bacillus proteus Morphological variation of long rod. Light microscope. X 1 000. b Bacillus proteus Morphological variation of long rod. Electron microscope. X 6 500. c The same as b but under the electron microscope nucleoplasm. X 13 000. the reagent solution and then examined using an AC-920 hemocyte analyzer. Assay of Humoral Immunologic Function. A quantitative hemolysis spectrophotometry QHS method was used to determine the amount of hemoglobin released after hemolysis of RBC mediated by antibody-forming cells 3 . This amount expressed as OD value reflected the amount of antibody-forming cells in mice thus indicating the humoral immunologic function of the mice. Table 37. Morphological variation of E. coli communis and pathogenic E. coli E. coli communis Pathogenic E. coli Generation 0 BO 1-6 7 19-12 I23 4-6 7-10 Variation Form of G- bacillus basically normal a little longer like diplo-bacillus became larger like round ball shape was normal but had deep particles in the bacteria . Table 38. Effect of MEBO on classification of leukocytes mean SE Group Animal Lymphocytes number Neutrophils 69.96 3.35 30.06 3.35 Control 8 MEBO 8 p value 69.59B6.50 50.61 6.50 0.01 Assay of Cellular Immunologic Function. Blood samples were taken from mice tails in the MEBO and the control groups respectively then smeared and stained according to the a-naphthalene acetate esterase ANAE method. These were then examined under the microscope. 100 lymphocytes were observed randomly. The percentage of ANAE-positive lymphocytes reflects the cellular immunologic function of the body. Histological Changes of Mice Skin after Treatment with MEBO. 0.5-cm2 skin tissue samples of mice taken from depilated areas of normal skin and skin treated with MEBO were fixed embedded stained with hematoxylin and eosin and then observed under the light microscope. Results Anti-Infection Effect of MEBO Morphological Variation of Bacteria. The results of morphological variation of E. coli communis .