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Báo cáo khoa học: "Prevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in questing Ixodes ricinus ticks in relation to the density of wild cervids"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Prevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in questing Ixodes ricinus ticks in relation to the density of wild cervids. | Acta Veterinaria Scandinavica BioMed Central Open Access Prevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in questing Ixodes ricinus ticks in relation to the density of wild cervids Olav Rosef 1 Algimantas Paulauskas2 and Jana Radzijevskaja2 Address Telemark University College B0 i Telemark Norway and 2Vytautas Magnus University Kaunas Lithuania Email Olav Rosef - olav.rosef@hit.no Algimantas Paulauskas - a.paulauskas@gmf.vdu.lt Jana Radzijevskaja - j.radzijevskaja@bs.vdu.lt Corresponding author Published 27 November 2009 Received 28 April 2009 r. J . .-.Ann J _ I r I I nz I- r I AIr I Accepted 27 November 2009 Acta Veterinaria Scandinavica 2009 51 47 doi 10.1186 1751-0147-51 -47 This article is available from http www.actavetscand.com content 51 1 47 2009 Rosef et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum have been considered as pathogens in animals and humans. The role of wild cervids in the epidemiology is not clear. We analyzed questing Ixodes ricinus ticks collected in spring for these pathogens from sites with high Fjelloyvsr and Strom and low density Tjore Hinnebu and Jomfruland of wild cervids to study the spread of the pathogens in questing ticks. Methods For detection of Anaplasma phagocytophilum a 77-bp fragment in the msp2 gene was used. Detection of Borrelia burgdorferi sensu lato was performed using the FL6 and FL7 primers according to sequences of conserved regions of the fla gene. The OspA gene located on the linear 49-kb plasmid was used as target in multiplex PCR for genotyping. Genospecies-specific primers were used in the PCR for Borrelia burgdorferi sensu stricto B. afzelii and B. .