Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa học " Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris "

The water-soluble crude polysaccharides obtained from the liquid culture broth of Cordyceps militaris by ethanol precipitation were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in three polysaccharide fractions that were termed CPSN Fr I, CPSN Fr II, and CPSN Fr III. Of the fractions, CPSN Fr II was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO) and expression of cytokines (IL-1 and TNF- ). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT-IR), and gas chromatography–mass spectrometry (GC–MS). Results. | Carbohydrate Polymers 82 2010 982-988 Contents lists available at ScienceDirect Carbohydrate Polymers journal homepage www.elsevier.com locate carbpol Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris Jong Seok Lee Jeong Seok Kwon Dong Pil Won Keun Eok Lee Won Cheol Shin Eock Kee Hong Department of Bioengineering and Technology Kangwon National University Chuncheon 200-701 Republic of Korea ARTICLE INFO ABSTRACT Article history Received 8 January 2010 Received in revised form 24 May 2010 Accepted 15 June 2010 Available online 19 June 2010 Keywords Cordyceps militaris Immunostimulating polysaccharide Macrophage activation Random coil conformation The water-soluble crude polysaccharides obtained from the liquid culture broth of Cordyceps militaris by ethanol precipitation were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in three polysaccharide fractions that were termed CPSN Fr I CPSN Fr II and CPSN Fr III. Of the fractions CPSN Fr II was able to upregulate the functional events mediated by activated macrophages such as production of nitric oxide NO and expression of cytokines IL-1 p and TNF-a . Its structural characteristics were investigated by a combination of chemical and instrumental analyses including methylation reductive cleavage acetylation Fourier transform infrared spectroscopy FT-IR and gas chromatography-mass spectrometry GC-MS . Results indicate that CPSN Fr II was a 1 6-branched-glucogalactomannan that had a molecular weight of 36 kDa. The configuration of the p-linkage and random coil conformation of CPSN Fr II were confirmed using a Fungi-Fluor kit and congo red reagent respectively. 2010 Elsevier Ltd. All rights reserved. 1. Introduction Most if not all basidiomycetes mushrooms have biologically active polysaccharides in the fruiting body culture broth and cultured mycelia. Polysaccharides .