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Báo cáo sinh học: "Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study. | ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS Slinger et al. Annals of Clinical Microbiology and Antimicrobials 2011 10 33 http www.ann-clinmicrob.eom content 10 1 33 RESEARCH Open Access Rapid PCR detection of group a streptococcus from flocked throat swabs A retrospective clinical study 1 2 1 1 3 1 Robert Slinger David Goldfarb Derek Rajakumar loana Moldovan Nicholas Barrowman Ronald Tam and Francis Chan1 Abstract Background Rapid diagnosis of GAS pharyngitis may improve patient care by ensuring that patients with GAS pharyngitis are treated quickly and also avoiding unnecessary use of antibiotics in those without GAS infection. Very few molecular methods for detection of GAS in clinical throat swab specimens have been described. Methods We performed a study of a laboratory-developed internally-controlled rapid Group A streptococcus GAS PCR assay using flocked swab throat specimens. We compared the GAS PCR assay to GAS culture results using a collection of archived throat swab samples obtained during a study comparing the performance of conventional and flocked throat swabs. Results The sensitivity of the GAS PCR assay as compared to the reference standard was 96.0 95 CI 90.1 to 98.4 specificity 98.6 95 CI 95.8 to 99.5 positive predictive value PPV 96.9 95 CI 91.4 to 99.0 and negative predictive value NPV of 98.1 95 CI 95.2 to 99.2 . For conventional swab cultures sensitivity was 96.0 95 CI 90.1 to 98.4 specificity 100 95 CI 98.2 to 100 PPV 100 95 CI 96.1 to 100 and NPV 98.1 95 CI 95.2 to 99.3 Conclusions In this retrospective study the GAS PCR assay appeared to perform as well as conventional throat swab culture the current standard of practice. Since the GAS PCR assay including DNA extraction can be performed in approximately 1 hour prospective studies of this assay are warranted to evaluate the clinical impact of the assay on management of patients with pharyngitis. Keywords PCR rapid internally-controlled LCGreen Group A Streptococcus pharyngitis flocked