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Báo cáo y học: "Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine. | Ivanovska and Dimitrova Arthritis Research Therapy 2011 13 R44 http arthritis-research.eom content 13 2 R44 RESEARCH ARTICLE Open Access Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine Nina Ivanovska Petya Dimitrova Abstract Introduction Glucosamine is an amino-monosaccharide and precursor of glycosaminoglycans major components of joint cartilage. Glucosamine has been clinically introduced for the treatment of osteoarthritis but the data about its protective role in disease are insufficient. The goal of this study was to investigate the effect of long term administration of glucosamine on bone resorption and remodeling. Methods The effect of glucosamine on bone resorption and remodeling was studied in a model of collagenase-induced osteoarthritis CIOA . The levels of macrophage-inflammatory protein MIP -1a protein regulated upon activation normal T-cell expressed and secreted RANTES soluble receptor activator of nuclear factor kappa-B ligand RANKL tumor necrosis factor TNF -a and interleukin IL -6 4 and 10 in synovial fluid were measured by enzyme-linked immunosorbent assay ELISA . Cell populations in synovial extracts and the expression of RANKL of receptors for TNF-a TNF-aR and interferon y IFN-yR on clusters of differentiation CD three positive T cells were analyzed by flow cytometry. Transforming growth factor TGF - 33 bone morphogenetic protein BMP -2 phosphorylated protein mothers against decapentaplegic homolog 2 pSMAD-2 RANKL and Dickkopf-1 protein DKK-1 positive staining in CIOA joints were determined by immunohistochemistry. Results The administration of glucosamine hydrochloride in CIOA mice inhibited loss of glycosaminoglycans GAGs and proteoglycans PGs in cartilage bone erosion and osteophyte formation. It decreased the levels of soluble RANKL and IL-6 and induced IL-10 increase in the CIOA joint fluids. Glucosamine limited the number of CD11b positive Ly6G neutrophils and RANKL positive .