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Báo cáo y học: "yclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Cyclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ – prostaglandin E2 dependent proliferation of growth plate chondrocytes. | Available online http arthritis-research.eom content 8 3 R78 Research article Cyclooxygenases and prostaglandin E2 receptors in growth plate chondrocytes in vitro and in situ - prostaglandin E2 dependent proliferation of growth plate chondrocytes Christoph Brochhausen1 Pia Neuland2 C James Kirkpatrick1 Rolf M Nusing3 and Gunter Klaus2 Institute of Pathology Johannes Gutenberg-University Mainz Germany 2Department of Pediatrics Philipps-University Marburg Germany institute of Clinical Pharmacology Johann Wolfgang Goethe-University Frankfurt Main Germany Corresponding author Gunter Klaus klaus@med.uni-marburg.de Received 26 Aug 2005 Revisions requested 28 Sep 2005 Revisions received 16 Mar 2006 Accepted 28 Mar 2006 Published 28 Apr 2006 Arthritis Research Therapy 2006 8 R78 doi 10.1186 ar1 948 This article is online at http arthritis-research.com content 8 3 R78 2006 Brochhausen et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Prostaglandin E2 PGE2 plays an important role in bone development and metabolism. To interfere therapeutically in the PGE2 pathway however knowledge about the involved enzymes cyclooxygenases and receptors PGE2 receptors is essential. We therefore examined the production of PGE2 in cultured growth plate chondrocytes in vitro and the effects of exogenously added PGE2 on cell proliferation. Furthermore we analysed the expression and spatial distribution of cyclooxygenase COX -1 and COX-2 and PGE2 receptor types EP1 EP2 EP3 and EP4 in the growth plate in situ and in vitro. PGE2 synthesis was determined by mass spectrometry cell proliferation by DNA 3H -thymidine incorporation mRNA expression of cyclooxygenases and EP receptors by RT-PCR on cultured cells and in homogenized growth