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Báo cáo y học: "Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen-induced arthritis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài:Local expression of matrix metalloproteinases, cathepsins, and their inhibitors during the development of murine antigen-induced arthritis. | Available online http arthritis-research.eom content 7 1 R1 74 Research article Local expression of matrix metalloproteinases cathepsins and their inhibitors during the development of murine antigen-induced arthritis Uta Schurigt1 Nadine Stopfel2 Marion Huckel1 Christina Pfirschke1 Bernd Wiederanders2 and Rolf Brauer1 Institute of Pathology Friedrich Schiller University Jena Germany institute of Biochemistry I Friedrich Schiller University Jena Germany Corresponding author Uta Schurigt Uta.Schurigt@med.uni-jena.de Received 7 Jun 2004 Revisions requested 3 Sep 2004 Revisions received 23 Sep 2004 Accepted 26 Oct 2004 Published 1 0 Dec 2004 Arthritis Res Ther 2005 7 R174-R188 DOI 10.1186 ar1466 2004 Schurigt et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Cartilage and bone degradation observed in human rheumatoid arthritis RA are caused by aberrant expression of proteinases resulting in an imbalance of these degrading enzymes and their inhibitors. However the role of the individual proteinases in the pathogenesis of degradation is not yet completely understood. Murine antigen-induced arthritis AIA is a well-established animal model of RA. We investigated the time profiles of expression of matrix metalloproteinase MMP cathepsins tissue inhibitors of matrix metalloproteinases TIMP and cystatins in AIA. For primary screening we revealed the expression profile with Affymetrix oligonucleotide chips. Realtime polymerase chain reaction PCR analyses were performed for the validation of array results for tests of more RNA samples and for the completion of the time profile. For the analyses at the protein level we used an MMP fluorescence activity assay and zymography. By a combination of oligonucleotide .