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Báo cáo y học: "The synovial proteome: analysis of fibroblast-like synoviocytes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The synovial proteome: analysis of fibroblast-like synoviocytes. | Available online http arthritis-research.eom content 6 2 R161 Research article Open Access The synovial proteome analysis of fibroblast-like synoviocytes Kumar Dasuri1 Mihaela Antonovici2 Keding Chen1 Ken Wong1 Kenneth Standing2 3 Werner Ens2 3 Hani El-Gabalawy1 and John A Wilkins1 2 3 1 Rheumatic Diseases Research Laboratory University of Manitoba Winnipeg Canada 2Manitoba Centre for Proteomics Department of Medicine University of Manitoba Winnipeg Canada 3Time of Flight Laboratory Department of Physics and Astronomy University of Manitoba Winnipeg Canada Corresponding author John A Wilkins e-mail jwilkin@cc.umanitoba.ca Received 17 Dec 2003 Revisions requested 13 Jan 2004 Revisions received 13 Jan 2004 Accepted 21 Jan 2004 Published 16 Feb 2004 Arthritis Res Ther 2004 6 R161-R168 DOI 10.1186 ar1153 2004 Dasuri et al. licensee BioMed Central Ltd Print ISSN 1478-6354 Online ISSN 1478-6362 . This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial FLS cells derived from the synovia of rheumatoid arthritis patients. The cellular proteins were separated by two-dimensional polyacrylamide gel electrophoresis and the in-gel digested proteins were analyzed by matrix-assisted laser desorption ionization mass spectrometry. A total of 368 spots were examined and 254 identifications were made. The studies identified a number of proteins that have been implicated in the normal or pathological FLS function e.g. uridine diphosphoglucose dehydrogenase galectin 1 and galectin 3 or that have been characterized as potential autoantigens in rheumatoid arthritis e.g. BiP colligin HC gp-39 . A novel uncharacterized protein product of chromosome 19 open reading frame 10 was also detected as an apparently major component of FLS .