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báo cáo hóa học:" Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model | Dewan et al. Journal of Orthopaedic Surgery and Research 2010 5 58 http www.josr-online.eom content 5 1 58 JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH RESEARCH ARTICLE Open Access Assessing mechanical integrity of spinal fusion by in situ endochondral osteoinduction in the murine model 1 1 1 1 22 Ashvin K Dewan Rahul A Dewan Nathan Calderon Angie Fuentes ZaWaunyka Lazard Alan R Davis Michael Heggeness1 John A Hipp1 Elizabeth A Olmsted-Davis2 Abstract Background Historically radiographs micro-computed tomography micro-CT exams palpation and histology have been used to assess fusions in a mouse spine. The objective of this study was to develop a faster cheaper reproducible test to directly quantify the mechanical integrity of spinal fusions in mice. Methods Fusions were induced in ten mice spine using a previously described technique of in situ endochondral ossification harvested with soft tissue and cast in radiolucent alginate material for handling. Using a validated software package and a customized mechanical apparatus that flexed and extended the spinal column the amount of intervertebral motion between adjacent vertebral discs was determined with static flexed and extended lateral spine radiographs. Micro-CT images of the same were also blindly reviewed for fusion. Results Mean intervertebral motion between control non-fused spinal vertebral discs was 6.1 0.2 during spine flexion extension. In fusion samples adjacent vertebrae with less than 3.5 intervertebral motion had fusions documented by micro-CT inspection. Conclusions Measuring the amount of intervertebral rotation between vertebrae during spine flexion extension is a relatively simple cheap 100 clinically relevant and fast test for assessing the mechanical success of spinal fusion in mice that compared favorably to the standard micro-CT. Background Spinal fusion is a common surgical procedure used to manage a variety of disorders. In 2001 over 50 of all inpatient lumbar spine operations other than those