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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
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Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Intravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head. | Int. J. Med. Sci. 2011 8 74 International Journal of Medical Sciences 2011 8 1 74-83 Ivyspring International Publisher. All rights reserved. Research Paper Intravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head Zhang-hua Li 1 x Wen Liao2 Xi-long Cui 1 Qiang Zhao 3 Ming Liu 1 You-hao Chen 1 Tian-shu Liu 1 Nong-le Liu 3 Fang Wang 3 Yang Yi 4 Ning-sheng Shao 3 1. Department of Orthopaedics Renmin Hospital of Wuhan University Wuhan 430060 China. 2. Department of Orthopedics Affiliated Hospital of Hebei University Baoding 071000 China. 3. Laboratory of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing 100850 China. 4. College of Health Science Wuhan Institute of Physical Education Wuhan 430079 China. Zhang-hua Li and Wen Liao contributed equally to this work. x Corresponding author Zhang-hua Li Tel 8627-88041911-82209 Email li1663@yeah.net Received 2010.08.11 Accepted 2011.01.01 Published 2011.01.09 Abstract In this study we investigated the feasibility and safety of intravenous transplantation of allogeneic bone marrow mesenchymal stem cells MSCs for femoral head repair and observed the migration and distribution of MSCs in hosts. MSCs were labeled with green fluorescent protein GFP in vitro and injected into nude mice via vena caudalis and the distribution of MSCs was dynamically monitored at 0 6 24 48 72 and 96 h after transplantation. Two weeks after the establishment of a rabbit model of femoral head necrosis GFP labeled MSCs were injected into these rabbits via ear vein immunological rejection and graft versus host disease were observed and necrotic and normal femoral heads bone marrows lungs and livers were harvested at 2 4 and 6 w after transplantation. The sections of these tissues were observed under fluorescent microscope. More than 70 MSCs were successfully labeled with GFP at 72 h after labeling. MSCs were .