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báo cáo khoa học: " Effect of Chemokine Receptors CCR7 on Disseminated Behavior of Human T cell Lymphoma: clinical and experimental study"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effect of Chemokine Receptors CCR7 on Disseminated Behavior of Human T cell Lymphoma: clinical and experimental study | Yang et al. Journal of Experimental Clinical Cancer Research 2011 30 51 http www.jeccr.eom content 30 1 51 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Effect of Chemokine Receptors CCR7 on Disseminated Behavior of Human T cell Lymphoma clinical and experimental study Jing Yang1t Shengyi Wang2t Guofan ZhaoH and Baocun S .r Abstract Background The expression of chemokine receptors CCR7 has been studied in relation to tumor dissemination and poor prognosis in a limited number of cancers. No such studies have been done on CCR7 expression in nonHodgkin s lymphoma T-NHL . Our aim in this paper is to investigate the association between CCR7 expression and progression and prognosis of T-NHL. Methods 1 Analysis of clinical data The specimens were obtained from 41 patients with T-NHL and 19 patients with lymphoid hyperplasia. Their corresponding clinicopathologic data were also collected. The expression levels of CCR7 MMP-2 and MMP-9 were examined by immunohistochemical staining. 2 Human T-NHL cell lines Hut 78 cutaneous T-cell lymphoma and Jurkat adult T-cell leukemia lymphoma were cultured. The invasiveness of the two cell lines were measured with a Transwell invasion assay and then used to study the effects of chemokine receptors on T-NHL invasion and the underlying molecular mechanism. The transcript and expression of CCR7 were evaluated using RT-PCR and western blotting. Results 1 The higher CCR7 and MMP-9 expression ratios were significantly associated with multiple lesions and higher stage III IV. Moreover a positive correlation was observed between CCR7 and MMP-9 expression. 2 The Hut 78 cell line was more invasive than the Jurkat cells in the Transwell invasion assay. The transcript and expression levels of CCR7 were significantly higher in Hut78 than that of Jurkat cell line. The T-NHL cell lines were co-cultured with chemokine CCL21 which increased the invasiveness of T-NHL cell. The positive association between CCL21 concentration and .