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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury | Zhang et al. Journal of Neuroinflammation 2011 8 118 http www.jneuroinflammation.eom content 8 1 118 JJOURNAL OF. NEUROINFLAMMATION RESEARCH Open Access Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury 1 1 2 3 1 1 1 Qing-Hong Zhang Qi Chen Jia-Rui Kang Chen Liu Ning Dong Xiao-Mei Zhu Zhi-Yong Sheng and Yong-Ming Yao1 4 Abstract Background Burn survivors develop long-term cognitive impairment with increased inflammation and apoptosis in the brain. Gelsolin an actin-binding protein with capping and severing activities plays a crucial role in the septic response. We investigated if gelsolin infusion could attenuate neural damage in burned mice. Methods Mice with 15 total body surface area burns were injected intravenously with bovine serum albumin as placebo 2 mg kg or with low 2 mg kg or high doses 20 mg kg of gelsolin. Samples were harvested at 8 24 48 and 72 hours postburn. The immune function of splenic T cells was analyzed. Cerebral pathology was examined by hematoxylin eosin staining while activated glial cells and infiltrating leukocytes were detected by immunohistochemistry. Cerebral cytokine mRNAs were further assessed by quantitative real-time PCR while apoptosis was evaluated by caspase-3. Neural damage was determined using enzyme-linked immunosorbent assay of neuron-specific enolase NSE and soluble protein-100 S-100 . Finally cerebral phospho-ERK expression was measured by western blot. Results Gelsolin significantly improved the outcomes of mice following major burns in a dose-dependent manner. The survival rate was improved by high dose gelsolin treatment compared with the placebo group 56.67 vs. 30 . Although there was no significant improvement in outcome in mice receiving low dose gelsolin 30 survival time was prolonged against the placebo control 43.1 4.5 h vs. 35.5 5.0 h P 0.05 . Burn-induced T cell suppression was greatly alleviated by high dose gelsolin treatment. Concurrently cerebral .