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Handbook of Microbiological Media, Fourth Edition part 124

Handbook of Microbiological Media, Fourth Edition part 124 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Motility Test and Maintenance Medium 1225 Use For the detection of motility in Gram-negative nonfermentative bacteria. Motility Nitrate Medium Buffered Composition per liter Peptone.5.0g Galactose.5.0g Agar.3.0g Beef extract.3.0g Na2HPO4.2.5g KNO3.1.0g Glycerin.5.0mL pH 7.3 0.1 at 25 C Preparation of Medium Add components except agar to dis-tilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Add agar. Gently heat until boiling. Distribute into tubes in 11.0mL volumes. Autoclave for 15 min at 15 psi pressure-121 C. If not used within 4 hr heat tubes to 100 C for 10 min. Use For the cultivation and observation of the motility of Clostridium perfringens. Motility Sulfide Medium Composition per liter Gelatin.80.0g Proteose peptone.10.0g NaCl.5.0g Agar.4.0g Beef extract.3.0g Sodium citrate.2.0g L-Cystine.0.2g Ferrous ammonium citrate.0.2g pH 7.3 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes in 4-5.0mL volumes. Autoclave for 15 min at 10 psi pressure-116 C. Use For the determination of bacterial motility and the ability of bacteria to produce H2S from L-cystine. For the differentiation of Gramnegative bacteria of the Enterobacteriaceae. Motility Test HiVeg Medium Composition per liter Plant hydrolysate No. 1.10.0g Agar.5.0g NaCl.5.0g pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from Hi-Media. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure-121 C. Use For the detection of bacterial motility. Motility Test HiVeg Medium Edwards and Ewing HiVeg Medium Composition per liter Plant peptone.10.0g NaCl. 5.0g Agar.4.0g .