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Handbook of Microbiological Media, Fourth Edition part 3

Handbook of Microbiological Media, Fourth Edition part 3 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | AAHC Medium for YAK Clones 15 Nicotinamide adenine dinucleotide.0.25g Cocarboxylase.0.1g Guanine-HCl.0.03g Fe NO3 3.0.02g p-Aminobenzoic acid.0.013g Vitamin B12.0.01g Thiamine-HCl.3.0mg Preparation of CVA Enrichment Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. i -Cysteine-HCTH2O Solution Composition per 10.0mL L-Cysteine-HCl-H2O.0.4g Preparation of i -Cysteine-HCTH2O Solution Add l-cysteine-HCl-H2O solution to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Urea Solution Composition per 10.0mL Urea ultrapure.1.0g Preparation of Urea Solution Add urea to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Aseptically combine 160.0mL of cooled sterile agar base and 45.9mL of sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use For the cultivation and differentiation of Ureaplasma urealyti-cum from urine based on its ability to produce ammonia from urea. Bacteria that produce ammonia appear as golden to dark brown colonies. Also used for the cultivation of other Ureaplasma species. A 8B Agar Composition per 84.6mL Agar base.80.0mL Supplement solution.4.6mL pH 6.0 0.2 at 25 C Agar Base Composition per 165.0mL Pancreatic digest of casein.2.72g Agar.2.1g NaCl.0.8g Papaic digest of soybean meal.0.48g K2HPO4.0.4g Glucose.0.4g MnSO4-H2O.0.15g CaCl22H2O.0.03g Putrescine-2HCl.34.0mg Preparation of Agar Base Add components except agar to distilled deionized water and bring volume to 165.0mL. Adjust pH to 5.5. Add agar. Mix thoroughly. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Supplement Solution Composition per 4.6mL Horse serum unheated.1.0mL Fresh yeast extract solution.1.0mL Penicillin solution.1.0mL Urea solution.1.0mL L-Cysteine-HCl-H2O solution.0.5mL GHL tripeptide solution.0.1mL Preparation of Supplement Solution Aseptically combine