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Advances in Applied Biotechnology Part 5

Tham khảo tài liệu 'advances in applied biotechnology part 5', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Fermentation Processes Using Lactic Acid Bacteria Producing Bacteriocins for Preservation and Improving Functional Properties of Food Products 69 workers 1988 developed a system for heterologous expression using a bacteriocin-negative L. sakei Lb790 strain where into Lb790 introduced two plasmids allowed the production of various bacteriocins sakacin P pediocin PA-1 and piscicolin 61 at levels equal to or exceeding levels in correspondence with the wild type cultures. Cuozzo et al. 2000 investigated II b class bacteriocin lactocin 705 from L. casei CRL 705 and reported about the required of both two peptides presence for the inhibitory activity. The cloning expression and nucleotide sequence of the genes involved in the synthesis of pediocin PA-l was reported by Marugg et al. 1992 . The genes were cloned and expressed in E. coli and 5.6-kbp fragment from the plasmid was found to be necessary for the bacteriocin production. Hoover and co-workers 1989 noted about the surveyed 37 pediococci cultures for the antagonistic effect against eight List. monocytogenes strains and indicated that a bacteriocin effect of these LABs against List. monocytogenes may not be limited to a few industrial starter cultures. 15 strains containing the Lactobacillus Pediococcus Lactococcus and Leuconostoc genera were examined for the inhibition against eight strains of List. monocytogenes Harris et al. 1989 and only cell-free supernatants from Lactobacillus species UAL11 P. acidilactici PAC 1.0 and Leuconostoc species UAL14 were reported to inhibit all eight strains of List. monocytogenes. The addition of proteolytic enzymes caused the prevention of the inhibition. Ennahar et al. 1996 found out that Lactobacillus plantarum WHE 92 produce a bacteriocin identical to pediocin AcH from P. acidilactici H though pediocin AcH was produced more effectively in L. plantarum WHE 92 in the pH range of 5.0 to 6.0 as compared to P. acidilactici H. Moreover L. plantarum WHE 92 seems to have more effective