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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model | Sun et al. Journal of Translational Medicine 2011 9 118 http www.translational-medicine.eom content 9 1 118 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model Cheuk-Kwan Sun1 2t Chia-Hung Yen3t Yu-Chun Lin4 5 Tzu-Hsien Tsai5 Li-Teh Chang6 Ying-Hsien Kao7 Sarah Chua5 Morgan Fu5 Sheung-Fat Ko8 Steve Leu4 5 and Hon-Kan Yip4 5 Abstract Background This study tested the hypothesis that autologous transplantation of adipose-derived mesenchymal stem cells ADMSCs can effectively attenuate acute pulmonary ischemia-reperfusion IR injury. Methods Adult male Sprague-Dawley SD rats n 24 were equally randomized into group 1 sham control group 2 IR plus culture medium only and group 3 IR plus intravenous transplantation of 1.5 X 106 autologous ADMSCs at 1h 6h and 24h following IR injury . The duration of ischemia was 30 minutes followed by 72 hours of reperfusion prior to sacrificing the animals. Blood samples were collected and lungs were harvested for analysis. Results Blood gas analysis showed that oxygen saturation was remarkably lower whereas right ventricular systolic pressure was notably higher in group 2 than in group 3 all p 0.03 . Histological scoring of lung parenchymal damage was notably higher in group 2 than in group 3 all p 0.001 . Real time-PCR demonstrated remarkably higher expressions of oxidative stress as well as inflammatory and apoptotic biomarkers in group 2 compared with group 3 all p 0.005 . Western blot showed that vascular cell adhesion molecule VCAM -1 intercellular adhesion molecule ICAM -1 oxidative stress tumor necrosis factor-a and nuclear factor-KB were remarkably higher whereas NAD P H quinone oxidoreductase 1 and heme oxygenase-1 activities were lower in group 2 compared to those in group 3 all p 0.004 . Immunofluorescent staining demonstrated notably higher number of CD68 cells but significantly fewer