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Báo cáo khoa học: "Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats | J. Vet. Sci. 2001 2 1 43-46 JOURNAL OF Veterinary Science Flow cytometric evaluation on the age-dependent changes of testicular DNA contents in rats Chang Yong Yoon Choong Man Hong Yong-Yeon Cho Ji Young Song I Jin Hong Dae Hyun Cho Beom Jun Lee1 Hee Jong Song2 and Cheol Kyu Kim Department of Pathology National Institute of Toxicological Research Korea Food and Drug Administration Seoul 122-704 Korea 1 Safety Research Center Korea Testing and Research Institute for Chemical Industry Seoul 150-038 Korea Department of Infectious Diseases College of Veterinary Medicine Chonbuk National University Chonju 561-756 Korea An age-dependent cellular change of DNA contents in the testis of Sprague-Dawley rats was investigated by flow-cytometric method. Testicular cell suspensions at the age of 4 5 6 7 8 10 12 16 and 26 weeks were prepared and stained with propidium iodide. The relative proportions in the number of mature and immature haploid 1n diploid 2n S-phase and tetraploid 4n cells were calculated. The proportion in the number of mature haploid cells was sharply increased to the age of 10 weeks about 38 thereafter increased slightly to the level of 42 at the age of 26 weeks. The proportion of immature haploid cells was dramatically increased to the age of 6 weeks then maintained at the level of 20 to 30 thereafter. The proportion of diploid cells was 64 at the age of 4 weeks then decreased gradually through the age of 26 weeks. The proportion of S-phase cells was increased to the age of 4 weeks then maintained at a plateau level to the age of 26 weeks. The proportion of tetraploid cells were about 26 at the age of 4 weeks then decreased gradually to the age of 26 weeks. These results suggest that the proportions of testicular cells may depend on the age of the rat and that the flow cytometric method may be useful in the evaluation of the spermatogenic status with regard to accuracy and sensitivity. Key words flow cytometry SD rats testis DNA contents Introduction .