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Báo cáo sinh học: "Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection | Genetic Vaccines and Therapy BioMed Central Methodology Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection Christopher Bradburne Kelly Robertson 1 and Dzung Thach 2 Open Access Address 1Center for Bio Molecular Science and Engineering US Naval Research Laboratory Code 6900 4555 Overlook Ave SW Washington DC 20375 USA and 2NIAID NIH DHHS Bldg 33 Room 3W10A.6 33 North Drive MSC 3203 Bethesda Maryland 20892-3203 USA Email Christopher Bradburne - bradburne@cbmse.nrl.navy.mil Kelly Robertson - kelly.robertson@cbmse.nrl.navy.mil Dzung Thach - thachdc@niaid.nih.gov Corresponding author tEqual contributors Published II May 2009 Received 16 January 2009 Genetic Vaccines and Therapy 2009 7 6 doi 10.1 186 1479-0556-7-6 Accepted 1 1 May 2009 This article is available from http www.gvt-journal.com content 7 I 6 2009 Bradburne et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Nucleofection is an emerging technology for delivery of nucleic acids into both the cytoplasm and nucleus of eukaryotic cells with high efficiency. This makes it an ideal technology for gene delivery and siRNA applications. A 96-well format has recently been made available for high-throughput nucleofection however conditions must be optimized for delivery into each specific cell type. Screening each 96-well plate can be expensive and descriptions of methods and outcomes to determine the best conditions are lacking in the literature. Here we employ simple methods including cell counting microscopy viability and cytotoxicity assays to describe the minimal experimental methods required to optimize nucleofection conditions for a given cell line. Methods We comprehensively measured and analyzed the .