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Báo cáo hóa học: " Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients"

Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients | Ochsenreither et al. Journal of Translational Medicine 2010 8 35 http www.translational-medicine.eom content 8 1 35 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Comparison of T-cell receptor repertoire restriction in blood and tumor tissue of colorectal cancer patients Sebastian Ochsenreither 1 Alberto Fusi1 Susanne Wojtke1 Antonia Busse1 Natascha C Nussler2 Eckhard Thiel1 Ulrich Keilholz1 and Dirk Nagorsen 1 3 4 Abstract Several immunotherapeutic approaches rely on antigen-specific T-cells. Restrictions in the T-cell receptor TCR repertoire were reported as indicator of anti-tumor cytotoxic T-lymphocyte CTL response in various tumor entities. It is unclear yet whether a TCR restriction in peripheral blood mirrors the tumor compartment. We compared the expression of TCR VjB-families for the quantification of TCR repertoire alterations in blood and tissue samples from patients with colorectal carcinoma. Blood samples from patients with colorectal carcinoma and healthy volunteers and tissue samples of normal colonic mucosa and colorectal carcinoma were analyzed. Relative VjB-family quantification was performed based on quantitative reverse transcribed PCR. Standard deviation and average mean of the single families were determined. Two variables describing the degree of VjB-repertoire restriction were defined. Forty-eight blood samples and 37 tissue samples were analyzed. TCR repertoire restriction was higher in blood of tumor patients than in blood of healthy controls p 0.05 . No difference in the degree of TCR repertoire restriction was found between carcinoma and unaffected colon tissue. We found no corresponding elevated TCR families among the different compartments blood normal colon and carcinoma tissue of the same patient. In conclusion we observed a repertoire restriction in peripheral blood as well as in tumor tissue of cancer patients. However in tumor tissue repertoire alterations were comparable to normal mucosa suggesting compartment-specific .