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Báo cáo hóa học: " Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells | Rossi et al. Journal of Translational Medicine 2010 8 23 http www.translational-medicine.eom content 8 1 23 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia mean fluorescence intensity T B ratio versus percentage of positive cells 1 2 3 2 2 Francesca M Rossi Maria Ilaria Del Principe Davide Rossi Maria Irno Consalvo Fabrizio Luciano 11 11 3 1 Antonella Zucchetto Pietro Bulian Riccardo Bomben Michele Dal Bo Marco Fangazio Dania Benedetti Massimo Degan 1 Gianluca Gaidano3 Giovanni Del Poeta23 Valter Gattei1 3 Abstract Background ZAP-70 is an independent negative prognostic marker in chronic lymphocytic leukemia CLL . Usually its expression is investigated by flow cytometric protocols in which the percentage of ZAP-70 positive CLL cells is determined in respect to isotypic control ISO-method or residual ZAP-70 positive T cells T-method . These methods however beside suffering of an inherent subjectivity in their application may give discordant results in some cases. The aim of this study was to assess the prognostic significance of these methods in comparison with another in which ZAP-70 expression was evaluated as a Mean-Fluorescence-Intensity Ratio between gated T and CLL cells T B Ratio-method . Methods Cytometric files relative to ZAP-70 determination according to the three readouts were retrospectively reviewed on a cohort of 173 patients test set all with complete clinical and biological prognostic assessment and time-to-treatment TTT available. Findings were then validated in an independent cohort of 341 cases from a different institution validation set . Results The optimal prognostic cut-offs for ZAP-70 expression were selected at 11 ISO-method or 20 of positive cells T-method as well as at 3.0 T B Ratio-method in the test set these cut-offs yielded 66 60 and 73 ZAP-70 cases respectively. Univariate analyses resulted in a better separation of ZAP-70 vs. ZAP-70- CLL patients utilizing