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Immobilisation of DNA on Chips I

The unique practical properties of adsorption have promoted its extensive use in genetic analysis. The disadvantages of adsorption with respect to covalent immobilization are mainly that (1) nucleic acids may be readily desorbed from the substrate, and (2) base moieties may be unavailable for hybridization if they are bonded to the substrate in multiple sites [34]. However, the electrochemical detection strategy based on the intrinsic oxidation of DNA requires the DNA to be adsorbed in close contact with the electrochemical substrate by multi-point attachment. This multi-site attachment of DNA can be thus detrimental for its hybridization but is crucial for the detection based on its oxidation signals | 260 Topics in Current Chemistry Editorial Board V. Balzani A. de Meijere K. N. Houk H. Kessler J.-M. Lehn S. V. Ley S. L. Schreiber J. Thiem B. M. Trost F. Vogtle H. Yamamoto Topics in Current Chemistry Recently Published and Forthcoming Volumes Molecular Machines Volume Editor Kelly T. R. Vol. 262 2006 Contrast Agents III Volume Editor Krause W. Vol. 252 2005 Immobilisation of DNA on Chips II Volume Editor Wittmann C. Vol. 261 2005 Chalcogenocarboxylic Acid Derivatives Volume Editor Kato S. Vol. 251 2005 Immobilisation of DNA on Chips I Volume Editor Wittmann C. Vol. 260 2005 New Aspects in Phosphorus Chemistry V Volume Editor Majoral J.-P. Vol. 250 2005 Prebiotic Chemistry From Simple Amphiphiles to Protocell Models Volume Editor Walde P. Vol. 259 2005 Templates in Chemistry II Volume Editors Schalley C. A. Vogtle F. Dotz K. H. Vol. 249 2005 Supramolecular Dye Chemistry Volume Editor Wurthner F. Vol. 258 2005 Templates in Chemistry I Volume Editors Schalley C. A. Vogtle F. Dotz K. H. Vol. 248 2004 Molecular Wires From Design to Properties Volume Editor De Cola L. Vol. 257 2005 Collagen Volume Editors Brinckmann J. Notbohm H. Muller P. K. Vol. 247 2005 Low Molecular Mass Gelators Design Self-Assembly Function Volume Editor Fages F. Vol. 256 2005 New Techniques in Solid-State NMR Volume Editor Klinowski J. Vol. 246 2005 Anion Sensing Volume Editor Stibor I. Vol. 255 2005 Functional Molecular Nanostructures Volume Editor Schluter A. D. Vol. 245 2005 Organic Solid State Reactions Volume Editor Toda F. Vol. 254 2005 Natural Product Synthesis II Volume Editor Mulzer J. Vol. 244 2005 DNA Binders and Related Subjects Volume Editors Waring M. J. Chaires J. B. Vol. 253 2005 Natural Product Synthesis I Volume Editor Mulzer J. Vol. 243 2005 Immobilisation of DNA on Chips I Volume Editor Christine Wittmann With contributions by S. Alegret I. J. Bruce A. del Campo A. Guiseppi-Elie T. Kawasaki L. Lingerfelt F. Luderer Y. Okahata D. V. Nicolau M. I. Pividori P. D. Sawant U. .