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Báo cáo khoa học: Mitochondrial Ca2+ sequestration and precipitation revisited

The ability of mitochondria to sequester and retain divalent cations in the form of precipitates consisting of organic and inorganic moieties has been known for decades. Of these cations, Ca 2+ has emerged as a major player in both signal transduction and cell death mechanisms, and, as a conse-quence, the importance of mitochondria in these processes was soon recog-nized. | MINIREVIEW Mitochondrial Ca2 sequestration and precipitation revisited Christos Chinopoulos and Vera Adam-Vizi Department of MedicalBiochemistry Semmelweis University NeurobiochemicalGroup Hungarian Academy of Sciences Budapest Hungary Keywords adenine nucleotides Ca2 uniporter complexation electron microscopy Na Ca2 exchanger phosphocitrate polyphosphate precipitation thermodynamics uncoupler Correspondence C. Chinopoulos V. Adam-Vizi Department of MedicalBiochemistry Semmelweis University Budapest Hungary Fax 361 2670031 Tel 361 4591500 ext. 60024 361 2662773 E-mail chinopoulos.christos@eok.sote.hu veronika.adam@eok.sote.hu Received 9 February 2010 revised 19 May 2010 accepted 22 June 2010 The ability of mitochondria to sequester and retain divalent cations in the form of precipitates consisting of organic and inorganic moieties has been known for decades. Of these cations Ca2 has emerged as a major player in both signal transduction and cell death mechanisms and as a consequence the importance of mitochondria in these processes was soon recognized. Early studies showed considerable effort in identifying the mechanisms of Ca2 sequestration precipitation and release by uncouplers of oxidative phosphorylation however relatively little information was obtained and these processes were eventually taken for granted. Here we re-examine a the thermodynamic aspects of mitochondrial Ca2 uptake and release b the insufficiently explained effect of uncouplers in inducing mitochondrial Ca2 release c the thermodynamic effects of exogenously added adenine nucleotides on mitochondrial Ca2 uptake capacity and precipitate formation and d the elusive nature of the Ca2 -phosphate precipitates formed in the mitochondrial matrix. doi 10.1111 j.1742-4658.2010.07755.x Why is it important to address Ca2 sequestration and precipitation Isolated mitochondria from a variety of sources exhibit a finite capacity to accumulate and retain divalent cations including Ca2 1 2 . This capacity .