Đang chuẩn bị liên kết để tải về tài liệu:
Study of silanized nanostructures with immobilized fumarase for production of L-malate
Đang chuẩn bị nút TẢI XUỐNG, xin hãy chờ
Tải xuống
The conversion of fumaric acid into L-malate by fumarase immobilized on silanized nanostructures was analyzed experimentally. The enzyme was bound to the silanized nanostructures. We carried out scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) analysis, zeta size analysis and surface area calculation for the characterization of the nanostructures. The effect of initial enzyme concentration and pH on immobilization procedure were investigated and the change of Michaelis-Menten constants (Km and Vmax) with immobilization was examined. |