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Protein ion-exchange chromatography on a biomacromolecule-immobilized monolithic cryogel

An efficient and inexpensive monolithic stationary phase (PHEMA-HA) has been prepared through an easy process comprising addition of humic acid (HA) to a mixture of 2-hydroxyethyl methacrylate (HEMA) and N,N’- methylenebisacrylamide (MBAAm) and subsequent radical-polymerization at –20 ◦ C. The prepared monolithic material was characterized in terms of various techniques and methods such as elemental analysis, FTIR spectroscopy, scanning electron microscopy, mercury porosimetry, hydrolytic stability tests, pHpzc measurements, water-holding capacity, and water permeability. The amount of HA incorporated into the structure was calculated as 45 mg/g from the elemental analysis results. |