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Comparison of RNA isolation methods on RNA-Seq: Implications for differential expression and meta-analyses

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The increasing number of transcriptomic datasets has allowed for meta-analyses, which can be valuable due to their increased statistical power. However, meta-analyses can be confounded by so-called “batch effects,” where technical variation across different batches of RNA-seq experiments can clearly produce spurious signals of differential expression and reduce our power to detect true differences. |

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Comparison of RNA isolation methods on RNA-Seq: Implications for differential expression and meta-analyses

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